What is the difference between GFP and mCherry?
As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish. Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy.
What wavelength is mCherry excited at?
mCherry has a single photon excitation peak at 587 nm and an emission peak at 610 nm .
What is GFP mCherry?
Background. Fluorescent proteins are powerful molecular biology tools that have been used to study the subcellular dynamics of proteins within live cells for well over a decade. Two fluorescent proteins commonly used to enable dual protein labelling are GFP (green) and mCherry (red).
What color is mCherry?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed.
What is the molecular weight of mCherry?
mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).
What channel is mCherry?
It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.
How stable is mCherry?
mCherry is monomeric and indeed seems to be quite stable (as for photobleaching), yet it has only about 50% of brightness compared to EGFP. This means that you actually might need to use more laser power/longer exposure and that at the end might contribute to photobleaching.
What is mCherry marker?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004).