What is HuH-7 cell line?
HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.
What are human hepatoma cells?
HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions.
How do you maintain a HuH-7 cell?
Subculture Protocol for HuH-7 Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%. Do not allow cell concentration to exceed 90% confluency, otherwise cells will decrease proliferation rates. The doubling time of HuH-7 cells is 24 hours.
What are Huh7 cells used for?
Huh-7 Origin and Sato, J. Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it is often used as a model to investigate hepatoma and HCV. This cell line can be used for an HCV replicon system, which allows for an in vitro production of infectious HCV particles and development of drugs that combat HCV.
What is the difference between HepG2 and Huh7?
In this way, HepG2 cells carry wild-type p53, whereas Hep3B and Huh7 cells have null and point mutations at p53 codon 220 respectively. Hep G2 cells mostly used for research to find level of cytotoxicity and cellular metabolism and drug metabolism in the induced cell line.
What is cell line hepatocellular carcinoma?
Hepatocellular carcinoma (HCC) cell lines are useful in vitro models for the study of primary HCCs. Because cell lines acquire additional mutations in culture, it is important to understand to what extent HCC cell lines retain the genetic landscapes of primary HCCs.
How do I revive my cell line?
The subsequent recovery of cryopreserved cells is straightforward: Cells are thawed rapidly in a water bath at 37°C, removed from the freeze-medium by gentle centrifugation and/or diluted with growth medium, and seeded in a culture vessel in complete growth medium.
How do you thaw HuH-7 cells?
- Pre-warm growth medium in 37 °C water bath.
- Thaw the cells in a 37 °C water bath (not completely, there should be a small visible ice clump inside the tube)
- Transfer the cells into a 15 ml centrifuge tube containing 9 ml pre-warmed growth medium.