What concentration does sodium azide use?
To prevent microbial contamination, sodium azide can be added to an antibody preparation for example to a final concentration of 0.02% (w/v) but if you are staining or treating live cells with antibodies, or if using antibodies for in vivo studies, be sure to use preparations that do not contain sodium azide.
Is sodium azide a preservative?
Sodium azide is used as a chemical preservative in hospitals and laboratories.
How do you make a 1% sodium azide solution?
1 M Sodium Azide (Na Azide) For a 1 M solution, dissolve 6.5 g of Na azide (m.w. 65.02) in 100 ml of dis- tilled H2O.
How much azide do you add to antibodies?
Antibodies of the IgG3 isotype are unique in their tendency to form aggregates upon thawing and should always be stored at 4oC. To prevent microbial contamination, sodium azide can be added to an antibody preparation to a final concentration of 0.02% (w/v).
Why is sodium azide a preservative?
Introduction. Sodium azide (NaN3) is a highly water-soluble bacteriostatic preservative used to prevent bacterial contamination in aqueous laboratory reagents [1], especially in those containing proteins isolated from biological fluids.
Why do we add sodium azide to media?
To prevent microbial contamination, you can add sodium azide to an antibody solution to a final concentration of 0.02% (w/v).
At what concentration is sodium azide toxic?
Sodium azide is highly toxic. Ingestion of 100 to 200 mg can result in headache, respiratory distress, and diarrhea. Target organs are the central nervous system and the brain.
How do you store sodium azide solution?
Store sodium azide in a cool, dry area away from heat and open flames. Separate it from incompatible material such as halogenated hydrocarbons, metals, water, acids, and acid chlorides. Avoid contact with metal shelves or containers.
How do you make azide?
Preparation. Sodium azide is made industrially by the reaction of nitrous oxide, N 2O with sodium amide NaNH 2 in liquid ammonia as solvent: N 2O + 2 NaNH 2 → NaN 3 + NaOH + NH. Many inorganic azides can be prepared directly or indirectly from sodium azide.
How do you preserve antibodies?
Antibodies are proteins and should be kept cold (refrigerated, on ice, or frozen) when not in use. c. The more dilute the antibody is, the less stable. Therefore, it is good idea to store antibodies in concentrated form without dilution.
How do you calculate antibody dilutions?
For example; lets say you need a total 3 ml of your primary antibodies, which also means 3000 uL. Now you need them to be diluted as 1:1000, so simply divide 3000 / 1000 which is 3. So take 3 uL from your Primary antibodies stock vial and add into 3000 uL (3 mL) of PBS or any other diluent as per your choice.
What do you dissolve sodium azide in?
Sodium azide can be destroyed by treatment with nitrous acid solution: 2 NaN 3 + 2 HNO 2 → 3 N 2 + 2 NO + 2 NaOH.